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Testing Inks For Bacteria And Mold


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6 replies to this topic

#1 LindaM

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Posted 21 February 2015 - 23:45

Just saw this today - it's a way to know for sure if anything is growing in the inks that are being experimented with, without having to just 'wait and see'.

 

http://www.lotioncra...ioncrafter.html



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#2 amberleadavis

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Posted 22 February 2015 - 13:05

Wow, that's nifty. Thank you for sharing.


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#3 musicman123

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Posted 22 February 2015 - 16:41

Great find! I was originally envisioning some kind of machine that checks for signs of organic life (protein structures, simple and complex sugars), but this is a much more elegant and easy to obtain solution.

 


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#4 white_lotus

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Posted 22 February 2015 - 19:45

There are a number of these kinds of kits listed at ColeParmer. Basically $40 per 10 tests.

 

http://www.coleparme...gar/EW-99532-46

 

The thing is these tests are designed to be dipped in the liquid being tested. If you had a sterile qtip, you could rub the ink over the medium, but I don't know what that would do to the colony counts after incubation.

 

You can also do this if you make up some agar plates in a petri dish and use an inoculating loop then see if anything grows. But inks rarely have sugars for bacteria, molds, yeasts to consume.



#5 LindaM

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Posted 22 February 2015 - 21:00

The sterile swabs are included in the kit. Or you could sterilize a metal spoon and pour the ink over the sticks. How many colonies you get isn't the issue so much as the fact that there are colonies. SITB happens, and when you're deliberately adding unknown substances like luster dust to the ink, it'd be good to know that nothing grew in the ink before putting it in a pen.

 

To make up agar petri dishes right you need to follow sterile procedure, such as wiping down your workspace with alcohol, wearing gloves, keeping the agar sterile (hard to do without an autoclave), all that ritual. Otherwise the odds of a false positive go way up. Not to mention it's difficult to buy that stuff in small quantities. These kits just make it a lot more convenient.



#6 amberleadavis

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Posted 12 March 2015 - 22:48

I think it is super helpful. Thank you so much for the link.


Fountain pens are my preferred COLOR DELIVERY SYSTEM (in part because crayons melt in Las Vegas).

 

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#7 Brianm_14

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Posted 20 October 2018 - 21:08

Writing as a microbiologist, I'd say that this sort of kit is great for anyone without training in sterile technique and access to some sort of lab. If you follow the directions carefully, you should get reliable results.
Brian






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